Anna Kloc, Ph.D.
Post-doctoral training, United States Department of Agriculture – Plum Island Animal Disease Center and Yale University School of Medicine
Ph.D., Genetics, Stony Brook University
B.A., Biology, Hunter College
viral replication, vaccine design, immune response to viral infection
Viruses present a threat to both human and animal health, and they can have severe public health and economic consequences. Once a virus enters the cell it must rapidly amplify its genome and produce infectious progeny, while avoiding host defense mechanisms. Viral replication is a highly regulated process that involves the interaction between viral proteins and host proteins to manipulate different cellular pathways in favor of virus survival. At the same time, the infected organism activates an immune response to the virus to counteract the infection. The competition between the virus and the host immune response ultimately determines the outcomes of the viral disease. Understanding how the virus replicates in the host cell and at the same time is able to avoid the host immune system is critical for the design of both antiviral drug strategies and for vaccine development.
Our laboratory combines molecular biology, virology and biochemistry approaches to investigate viral replication in cells. We use molecular biology techniques to design replicons – genetically altered viral genomes – to identify determinants important for viral genome synthesis. Furthermore, we utilize yeast, Saccharomyces cerevisiae, to identify novel host proteins that may influence viral replication. We are very interested in the in-depth characterization of potential viral proteins that interact with host proteins to determine how we can better develop vaccines or new antiviral strategies to control viral infection.
Kloc A, Rai DK, Rieder E The Roles of Picornavirus Untranslated Regions in Infection and Innate Immunity. Frontiers in Microbiology, 2018 March 20;9:485
Kloc A, Diaz-San Segundo F, Schafer E, Rai DK, Keeney M, de los Santos T, Rieder E Foot-and-mouth disease virus 5’-terminal S fragment is required for replication and modulation of the innate immune response in host cells. Virology, 2017 Sep 26;512:132-143
Rai DK, Diaz-San Segundo F, Campagnola G, Keith A, Schafer EA, Kloc A, de Los Santos T, Peersen O, Rieder E Attenuation of Foot-and-Mouth Disease Virus by Engineering Viral Polymerase Fidelity. Journal of Virology, 2017 Jun12;91(15)
Medina GN, Knudsen GM, Greninger AL, Kloc A, Diaz-San Segundo F, Rieder E, Grubman MJ, DeRisi JL, de Los Santos T Interaction between FMDV Lpro and transcription factor ADNP is required for optimal viral replication. Virology, 2017 May;505:12-22
Rai DK, Lawrence P, Kloc A, Schafer E, Rieder E Analysis of the interaction between host factor Sam68 and viral elements during foot-and-mouth disease virus infection. Virology Journal, 2015 Dec 23;12(1):224
Kloc A, Ivanova N Chromatin and Pluripotency: the MYSTerious connection. Cell Stem Cell, 2012 Aug 3; 11(2):139-40
Zaratiegui M, Castel S, Irvine D, Kloc A, Ren J, Li F, de Castro E, Martin L, Chang AY, Goto D, Cande WZ, Antequera F, Acrangioli B, Martienssen RA RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II. Nature, 2011 Oct 16; 476(7371):135-8
Martienssen RA, Kloc A, Slotkin RK, Tanurdzić M Epigenetic inheritance and reprogramming in plants and fission yeast. Cold Spring Harbor Symposium Quantitative Biology, 2008;73:265-71
Kloc A and Martienssen R RNAi, heterochromatin and the cell cycle. Trends in Genetics, 2008 Oct; 24(10):511-7
Kloc A, Zaratiegui M, Nora E, Martienssen R RNA interference guides histone modification during the S phase of chromosomal replication. Current Biology, 2008 Apr 8;18(7) 490-5